Esteban: Check out Fig’s. 53 – 56, and afterwards in: The
Image Processing Handbook by John C. Russ, CRC Press, 1992. If you want, I can
scan the pages and email a pdf to you.
Regards
Tim
From: Confocal Microscopy
List [mailto:[hidden email]] On Behalf Of G. Esteban
Fernandez
Sent: Tuesday, December 09, 2008 1:26 PM
To: [hidden email]
Subject: Removing slit-scanning confocal line artifacts
Hello all,
We sometimes see vertical line artifacts in images acquired
with our slit-scanning confocal. The pattern is highly
reproducible and can be divided out with a "blank"
image. However, we sometimes have to filter out the artifacts
in Fourier space by masking with a thin horz. rectangle through the middle
of the FFT map, save the very center. Can someone please point out references where line artifacts have
been removed with a filter (not by dividing out), whether in Fourier space
or not?
Those
interested can see sample images and FFT mask at http://www.mediafire.com/?sharekey=7b16b66ca9129ce991b20cc0d07ba4d2a83703a20834b252
Thanks,
Esteban
--
G. Esteban Fernandez, Ph.D.
Associate Director
Molecular Cytology Core Facility
University of Missouri
120 Bond Life Sciences Center
Columbia, MO 65211
http://www.biotech.missouri.edu/mcc/
573-882-4895
573-884-9395 fax
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