http://confocal-microscopy-list.275.s1.nabble.com/Removing-slit-scanning-confocal-line-artifacts-tp1635395p1636775.html
>
> Hi Esteban, I can't give you a reference, unfortunately, but I can
> confirm that we had the same problem years ago with a Zeiss LSM5
> live; I wrote an automated pre-processing tool in IDL that removed
> the critical part from the FFT and did the inverse FFT for all
> images. The problem was eventually solved by Zeiss.
> Hope this helps,
> Zoltan
>
>
> On Tue, Dec 9, 2008 at 7:25 PM, G. Esteban Fernandez
> <
[hidden email]> wrote:
> Hello all,
>
> We sometimes see vertical line artifacts in images acquired with our
> slit-scanning confocal. The pattern is highly reproducibleand can
> be divided out with a "blank" image.However, we sometimes have
> tofilter out the artifacts in Fourier spaceby masking with a thin
> horz. rectangle through the middle of the FFT map, save the very
> center. Can someone please point out references where line artifacts
> have been removedwith a filter (not by dividing out), whether in
> Fourier space or not?
>
> Those interested can see sample images and FFT mask at
>
http://www.mediafire.com/?sharekey=7b16b66ca9129ce991b20cc0d07ba4d2a83> 703a20834b252
>
>
> Thanks,
> Esteban
>
> --
> G. Esteban Fernandez, Ph.D.
> Associate Director
> Molecular Cytology Core Facility
> University of Missouri
> 120 Bond Life Sciences Center
> Columbia, MO 65211
>
>
http://www.biotech.missouri.edu/mcc/>
> 573-882-4895
> 573-884-9395 fax
>
>
>
> --
>
> Zoltan Cseresnyes
> Facility manager, Imaging Suite
Joel B. Sheffield, Ph.D.