Re: Removing slit-scanning confocal line artifacts

Posted by Roman Veith on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Removing-slit-scanning-confocal-line-artifacts-tp1635395p1638281.html

Hi!

We had the same problem with a Zeiss LSM Live microscope. The problem
was first thought to be related to dust at the CCD line (and therefore
producing a vertical line, because one or more pixels are "covered").
The ZEISS service tried to solve the problem, it resulted at least in
some hardware exchange at the LIVE unit, so maybe the dust was not the
only porblem. Now it's better, but in certain cases one can still see
some lines. If the lines recently occured maybe it's really dust.

Regards,
Roman


G. Esteban Fernandez schrieb:

> Hello all,
>  
> We sometimes see vertical line artifacts in images acquired with our
> slit-scanning confocal.  The pattern is highly reproducible and can be
> divided out with a "blank" image.  However, we sometimes have
> to filter out the artifacts in Fourier space by masking with a thin
> horz. rectangle through the middle of the FFT map, save the very
> center.  Can someone please point out references where line artifacts
> have been removed with a filter (not by dividing out), whether in
> Fourier space or not?
>  
> Those interested can see sample images and FFT mask at
> http://www.mediafire.com/?sharekey=7b16b66ca9129ce991b20cc0d07ba4d2a83703a20834b252 
> <https://webmail.um.umsystem.edu/exchweb/bin/redir.asp?URL=http://www.mediafire.com/?sharekey=7b16b66ca9129ce991b20cc0d07ba4d2a83703a20834b252>
>  
> Thanks,
> Esteban
>
> --
> G. Esteban Fernandez, Ph.D.
> Associate Director
> Molecular Cytology Core Facility
> University of Missouri
> 120 Bond Life Sciences Center
> Columbia, MO  65211
>
> http://www.biotech.missouri.edu/mcc/
>
> 573-882-4895
> 573-884-9395 fax

--
--
Roman Veith
Diplom-Biologe

Institut für physikalische und
theoretische Chemie
AG Biophysikalische Chemie
Universität Bonn
Wegelerstraße 12
53115 Bonn

Tel-Nr.: 0228-733089
Fax-Nr.: 0228-739424

http://www.thch.uni-bonn.de/pc/kubitscheck/