>
> I have a LSM 510 with a 365 UV laser
(water cooled Ar laser). It's
> coupled to the scan head via a quartz
fiber. Inside the head is a
> (factory) UV collimator lens. The
objectives are all planNeo or Plan
> Apo. We have no "UV" (Ultrafluor)
lenses. The cost is considerable:
> The laser is a Coherent Enterprise II
system with a water-to-water
> heat exchanger. The laser was >$60k,
and is now obsolete. I have a
> warranty directly from Coherent. To
change your non-UV scan head
> would require sending back to the factory
(Germany, not the US) to
> replace the primary dichroic, add a second
fiber coupler, add the
> collimator, add the UV AOTF (factory-installed
at the output window
> of the Coherent), and whatever other internal
optics CZ requires.
> Given that the Enterprise is obsolete, you'll
probably have to go
> with a UV diode, but they're not cheap either, and
you still have the
> fiber and the AOTF to contend with.
>
>
Steve...
>>
>>
>>On 24/12/08 3:47 AM, "G. Esteban
Fernandez"
>><<>
[hidden email]>
wrote:
>>
>>Hello all,
>>
>>On our Zeiss LSM
510 META two-photon system we have a Coherent
>>Chameleon NIR laser
that may need major repair. Coherent has been
>>very good in
providing remote technical assistance to us but we're
>>at a point
where a service visit is required and we're not under
>>contract. Since we do more shallow imaging of UV dyes like
DAPI on
>>that system that two-photon imaging, we would replace the
NIR laser
>>with UV if it is more cost-effective than repairing the
NIR. Zeiss
>>says a switch from NIR to UV would require a major
(and pricey)
>>overhaul of the optics. I don't know what that
entails but it is
>>understandable given the huge difference in
wavelength. However, I
>>wonder how badly out of focus or
aberrated the UV image would
>>actually be with the optics that are
already in place if we put a UV
>>laser in there in place of the NIR;
we do have a UV laser dichroic.
>> Might it be good enough for at
least localizing nuclei? (I do know
>>how to do non-confocal UV
imaging on the system) Can people advise
>>on an NIR-to-UV swap
of lasers?
>>
>>Thanks,
>>Esteban
>
>
> --
>
____________________________________________________________________________
>
Steven E. Ruzin, Ph.D.
> Director, Biological Imaging Facility
> 381
Koshland Hall College of Natural Resources
> University of California
Berkeley CA 94720-3102
> 510-642-6602 510-642-4995
>
(fax)
>
http://microscopy.berkeley.edu