Re: mounting media

Posted by Janos Roszik on
URL: http://confocal-microscopy-list.275.s1.nabble.com/mounting-media-tp2113645p2116851.html

Hi Serra,

 

you can use any non-fluorescent media for mounting, except for anti-fade reagents which prevent the photobleaching of the acceptor dye. When we use formaldehyde for fixation at the end of the experiment, we leave it on the cells, otherwise, we use PBS or glycerol.
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<a onclick="return top.js.OpenExtLink(window,event,this)" href="http://www.biophys.dote.hu/accpbfret/" target="_blank">http://www.biophys.dote.hu/accpbfret/

 

Best regards,

Janos
 

On 1/5/09, Serra Akinturk <[hidden email]> wrote:

Dear all,

 

I would like to take your opinion about the mounting reagent that i should use for fixed cells in acceptor photobleaching experiments.

Which is the best mounting reagent for fixed cells in acceptor photobleaching experiments?

I will look forward to hear from you.

Thanks.

Serra