Hi,
I think any non-fluorescent media can be used except for antifade reagents which prevent the photobleaching of the acceptor. When we do formaldehyde fixation at the end of an experiment, we usually don't change it. Otherwise, we use PBS, glycerol or 1:1 PBS:glycerol.
If you need an efficient program for the analysis of images, I suggest our free ImageJ plugin:
<a onclick="return top.js.OpenExtLink(window,event,this)" href="http://www.biophys.dote.hu/accpbfret/" target="_blank">http://www.biophys.dote.hu/accpbfret/
Janos
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