Live cell FRET - Glass or Plastic?

Posted by Pablo German on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Live-cell-FRET-Glass-or-Plastic-tp2195385.html

Hi Everyone,

 

I'm in the process of doing FRET experiments using the pair CFP-YFP in live cells and I was wondering whether glass-bottomed dishes are better than plastic dishes for confocal imaging. HEK cells don't seem to want to attach to glass-bottomed dishes even when they are coated with poly-lysine, so using plastic dishes would make things easier.

 

Thanks in advance,

Pablo

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Pablo German

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