Re: Live cell FRET - Glass or Plastic?

Posted by vaishali kailaje on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Live-cell-FRET-Glass-or-Plastic-tp2195385p2196387.html

Hi,

Glass bottom plates are any time preferable than plastic bottom for live cell imaging at higher magnification as you will not face any problems while focusing as well as there would be no loss in intensity.

Regarding growing cells , we have experienced that cells adhere very well when serum containing medium is used rather than in serum free medium.Probably you could try the same.

Best Luck.

Vaishali



--- On Thu, 1/22/09, Pablo German <[hidden email]> wrote:

> From: Pablo German <[hidden email]>
> Subject: Live cell FRET - Glass or Plastic?
> To: [hidden email]
> Date: Thursday, January 22, 2009, 5:32 AM
> Hi Everyone,
>
> I'm in the process of doing FRET experiments using the
> pair CFP-YFP in live
> cells and I was wondering whether glass-bottomed dishes are
> better than
> plastic dishes for confocal imaging. HEK cells don't
> seem to want to attach
> to glass-bottomed dishes even when they are coated with
> poly-lysine, so
> using plastic dishes would make things easier.
>
> Thanks in advance,
> Pablo
>
> --
> Pablo German
>
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