Re: Live cell FRET - Glass or Plastic?

Posted by Csúcs Gábor on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Live-cell-FRET-Glass-or-Plastic-tp2195385p2196720.html

Hallo Pablo,

You may try the Ibidi chambers/slides although I have no idea whether
the are represented in the southern hemisphere. They have a thin plastic
bottom but concerning imaging you can reach nearly the same quality as
with glass.

Cheers    Gabor

No commercial interest.

> Hi Everyone,
>  
> I'm in the process of doing FRET experiments using the pair CFP-YFP in
> live cells and I was wondering whether glass-bottomed dishes are
> better than plastic dishes for confocal imaging. HEK cells don't seem
> to want to attach to glass-bottomed dishes even when they are coated
> with poly-lysine, so using plastic dishes would make things easier.
>  
> Thanks in advance,
> Pablo
>
> --
> Pablo German
>
> Plant and Food Research
> Private Bag 92169
> Auckland Mail Centre
> Auckland 1142
> New Zealand
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--
Gabor Csucs
Light Microscopy Centre, ETH Zurich
Schafmattstrasse 18, HPM F16
CH-8093, Zurich, Switzerland

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