Live cell Imaging with fluoview 1000

Posted by Jean-Pierre CLAMME-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Live-cell-Imaging-with-fluoview-1000-tp2242987.html


Hi,

I had my first experience  today with this system for live cell imaging and all my cells under the laser didn't make it safely (the one around were just fine).

So I'm curious to know if some of you used a fluoview 1000 for live cell imaging of hoechst or Fm4-64? If yes, what kind of range of power and aquisition repetition (z stack and repetition) did your cells take ? (I did a stack of 16 images in z every 2 minutes so around 1minute of imaging and 1 minute of resting)

Thanks,

JP


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Jean-Pierre CLAMME, PhD
Senior Scientist
Nitto Denko Technical
501 Via Del Monte
Oceanside, CA 92058
E-mail: [hidden email]
Phone: [phone]