Hi JP,
I have used an
FV1000 in the past to image live cells and larvae for 12-24 hours with no
bleaching/phototoxicity.This was done with laser power setting in the <10%
range using a multi ion argon laser and a 405nm solid state laser.
Can you supply some more information?
-
Which lasers do you have on your machine (Argpn Ion, Solid state
etc.)?
-
How long are you imaging for
-
What is the sample?
-
What media are you using
Cheers
Cam
Cameron J. Nowell
Microscopy Manager
Centre for Advanced Microscopy
Ludwig Institute for Cancer Research
PO Box 2008
Royal Melbourne Hospital
Victoria, 3050
AUSTRALIA
Office: +61 3 9341 3155
Mobile: +61422882700
Fax: +61 3 9341 3104
From: Confocal Microscopy List
[mailto:[hidden email]] On Behalf Of Jean-Pierre
CLAMME
Sent: Friday, 30 January 2009 2:32 PM
To: [hidden email]
Subject: Live cell Imaging with fluoview 1000
Hi,
I had my first
experience today with this system for live cell imaging and all my cells
under the laser didn't make it safely (the one around were just fine).
So I'm curious
to know if some of you used a fluoview 1000 for live cell imaging of hoechst or
Fm4-64? If yes, what kind of range of power and aquisition repetition (z stack
and repetition) did your cells take ? (I did a stack of 16 images in z every 2
minutes so around 1minute of imaging and 1 minute of resting)
Thanks,
JP
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Jean-Pierre CLAMME, PhD
Senior Scientist
Nitto Denko Technical
501 Via Del Monte
Oceanside, CA 92058
E-mail: [hidden email]
Phone: +760.435.7065
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