Re: Live cell Imaging with fluoview 1000

Posted by Naomi Book on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Live-cell-Imaging-with-fluoview-1000-tp2242987p2243590.html

Hi,

I have used the FV-1000 many times,  for long (up to 72 hours) live cell imaging, using HeLa cells, cos and other cell lines. I use 2 excitations 488 and 543. the cells survive with no problem. the LIS incubator is doing a great job.

 

Naomi Book

 

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Naomi Melamed-Book, PhD

Bio-Imaging Unit, Life Science Institute

Hebrew University, Jerusalem, Israel.

E-mail: [hidden email]

Phone: [phone]

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----- Original Message -----

From: [hidden email]

To: [hidden email]

Sent: Friday, January 30, 2009 5:31 AM

Subject: Live cell Imaging with fluoview 1000

Hi,

I had my first experience  today with this system for live cell imaging and all my cells under the laser didn't make it safely (the one around were just fine).

So I'm curious to know if some of you used a fluoview 1000 for live cell imaging of hoechst or Fm4-64? If yes, what kind of range of power and aquisition repetition (z stack and repetition) did your cells take ? (I did a stack of 16 images in z every 2 minutes so around 1minute of imaging and 1 minute of resting)

Thanks,

JP


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Jean-Pierre CLAMME, PhD
Senior Scientist
Nitto Denko Technical
501 Via Del Monte
Oceanside, CA 92058
E-mail: [hidden email]
Phone: [phone]

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