Posted by
mmodel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Live-cell-Imaging-with-fluoview-1000-tp2242987p2244886.html
You can set the threshold at your chosen value and check the "Limit to threshold" box in Set Measurements. Then Analyze - Measure.
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Steffen Dietzel
Sent: Friday, January 30, 2009 5:25 AM
To:
[hidden email]
Subject: percentile calculation, ImageJ or otherwise?
Dear all,
I have a stack of images (16bit) for which I need
some measurement of the average signal intensity,
to describe signal loss with increasing depth.
Since the particular signal is sort of repetitive
(Collagen matrix), I thought of just going for
the 5-percentile (5% of all pixels in an image
plane have the grayvalue X or higher).
ImageJ readily calculates the median
(50-percentile) but I didn't find an option for
other percentiles in the program or on the
plug-ins web page. It is probably easy to program
it if one knows how and I am sure others have
done it before but programming isn't one of my capabilities.
Other suggestions to solve this problems also would be welcome
Best regards
Steffen
--
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Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy
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[hidden email]