Re: Multi-user Imaging room plans?

Posted by Martin Wessendorf-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Multi-user-Imaging-room-plans-tp2264638p2265513.html

Dear Dr. Charlton--

Milton Charlton wrote:
> We are contemplating building a multi-user imaging room containing 3
> confocals, 2  2-photon confocals, 1-2 widefield scopes.  We would
> isolate parts of the room with curtains.
> Has anyone constructed such a room and can send plans and/or
> recommendations about construction, utilities, etc?  Photos might help too.
> Thanks for your help.

One suggestion: be very careful about how the air conditioning is set
up.  Depending upon (1) the temperature differential between ambient air
and the air conditioning outflow, and (2) the placement of the air
conditioning duct relative to your microscope, you can produce VERY
noticeable (--like 3 um) shifts in focus/stage position.  The resulting
3D reconstructions look pretty odd.

Martin
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455
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