Re: Multi-user Imaging room plans?

Posted by Donnelly, Tom on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Multi-user-Imaging-room-plans-tp2264638p2265894.html

Amen to that!!

I have seen huge drift XY and Z with poor temperature control.  An AC or
Heat vent blowing on the system can create havoc on imaging and
deconvolution.

We had a case that we named "dot boogie"  where a bead would "boogie"
out of the field of view (60x) and boogie back in depending if the AC
was blowing or not. Z was also "interesting".

Cheers,
Tom

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Martin Wessendorf
Sent: Tuesday, February 03, 2009 12:39 PM
To: [hidden email]
Subject: Re: Multi-user Imaging room plans?

Dear Dr. Charlton--

Milton Charlton wrote:
> We are contemplating building a multi-user imaging room containing 3
> confocals, 2  2-photon confocals, 1-2 widefield scopes.  We would
> isolate parts of the room with curtains.
> Has anyone constructed such a room and can send plans and/or
> recommendations about construction, utilities, etc?  Photos might help
too.
> Thanks for your help.

One suggestion: be very careful about how the air conditioning is set
up.  Depending upon (1) the temperature differential between ambient air
and the air conditioning outflow, and (2) the placement of the air
conditioning duct relative to your microscope, you can produce VERY
noticeable (--like 3 um) shifts in focus/stage position.  The resulting
3D reconstructions look pretty odd.

Martin
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455
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