Re: colocalization question

Posted by Julio Vazquez on
URL: http://confocal-microscopy-list.275.s1.nabble.com/colocalization-question-tp2272746p2272775.html

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Actually, many colocalization programs, including the imageJ plugin "Colocalization finder"  will tell you the percent of each channel that is colocalized. see for instance:

http://rsb.info.nih.gov/ij/plugins/colocalization-finder.html

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Julio Vazquez

Fred Hutchinson Cancer Research Center

Seattle, WA 98109-1024

http://www.fhcrc.org/

On Feb 4, 2009, at 5:05 PM, Jean-Pierre CLAMME wrote:

Hi,

When you do colocalization measurement (in image pro for example) between let say the red and a green channel the co-localization coefficient tells you how much red and green are co-localized.  But as much as I understood, it doesn't give you information on the fact that for example red is always co-localized with green but green is not always colocalized with red ? What kind of analysis  would I need to compare this between samples ?

Thank you in advance,

JP


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Jean-Pierre CLAMME, PhD
Senior Scientist
Nitto Denko Technical
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