Re: colocalization question

Posted by Jean-Pierre CLAMME-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/colocalization-question-tp2272746p2275873.html


Thank you all very much. Your inputs  helped a lot !

JP

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Jean-Pierre CLAMME, PhD
Senior Scientist
Nitto Denko Technical
501 Via Del Monte
Oceanside, CA 92058
E-mail: [hidden email]
Phone: [phone]


Confocal Microscopy List <[hidden email]> wrote on 02/05/2009 03:00:41 AM:

> Dear JP,

> You need to measure thresholded Manders coefficients (1 for red , 1
> for green!)

> search this list archives for my previous long posts on this topic.

>
> in short, use ImageJ or easier BioImageXD (both free)
> to calculate threshold using the methods of costes,
> then calculate the Manders coefficents 1 and 2
> then do the costes statistical significance test.

> Publish:
> thresholds, manders coefficents, statistical significance,
> 2D histogram . scatter plots with straight line fit and thresholds.

> You need to read 2 papers at least
> Costes et al
> and Manders et al.

> I will send them to you in a moment

> cheers

> Dan

>
> Begin forwarded message:

> >
> > Date:    Wed, 4 Feb 2009 17:05:30 -0800
> > From:    Jean-Pierre CLAMME <[hidden email]>
> > Subject: colocalization question
> >
> > This is a multipart message in MIME format.
> > --=_alternative 0005FF9888257554_=
> > Content-Type: text/plain; charset="US-ASCII"
> >
> > Hi,
> >
> > When you do colocalization measurement (in image pro for example)
> > between
> > let say the red and a green channel the co-localization coefficient
> > tells
> > you how much red and green are co-localized.  But as much as I
> > understood,
> > it doesn't give you information on the fact that for example red is
> > always
> > co-localized with green but green is not always colocalized with red ?
> > What kind of analysis  would I need to compare this between samples ?
> >
> > Thank you in advance,
> >
> > JP
> >
> >
> > - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
> > Jean-Pierre CLAMME, PhD
> > Senior Scientist
> > Nitto Denko Technical
> > 501 Via Del Monte
> > Oceanside, CA 92058
> > E-mail: [hidden email]
> > Phone: [phone]

> Dr. Daniel James White BSc. (Hons.) PhD
> Senior Microscopist / Image Processing and Analysis
> Light Microscopy Facility
> Max Planck Institute of Molecular Cell Biology and Genetics
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> 01307 DRESDEN
> Germany

>
> New Mobile Number!!!

> [phone] (German Mobile)
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> [hidden email]
> ( [hidden email] )