Re: Leica SP5 question
Posted by
Adrian Smith-6 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-25-X-Plan-Neofluor-Phase-3-objective-for-sale-tp2286535p2300890.html
Also, does the Mark & Find function work in Live Data in version 2?
When I had a quick look at in 1.8.2 it didn't seem like "mark and find" was available in Live Data mode? (or at least I couldn't see it).
Regards,
Adrian Smith
Centenary Institute, Sydney, Australia
On 10/02/2009, at 2:32 PM, Dr. Graham Wright wrote:
Hello Zoltan & listers,
You comments suggest that you are a regular user of the Leica Live Data Mode on the SP5... if so, I was wondering how you handle the data post-acquisition - to get back to individual time series from each of the different positions. We tend to use ImageJ and macros we have written to sort and concatenate the files, but I am interested in how others approach this.
Best wishes,
Graham
----- Original Message -----
Sent: Tuesday, February 10, 2009 6:36 AM
Subject: Re: Leica SP5 question
Aryeh,
If you upgrade to LAS AF version 2.0, you'll be able to open a second image window and observe previously acquired data. We very much like the new version anyway, e.g. we find the Mark and Find and Live Data Mode more stable than even with the 1.8.2, which was already pretty good. I hope this helps,
Zoltan
On Mon, Feb 9, 2009 at 10:31 PM, Aryeh Weiss
<[hidden email]> wrote:
When acquiring confocal data, we find it useful to have a previous experiment (usually an image acquired earlier during that session) open, while making the live acquisition. This allows an immediate visual comparison of the current result with a previous result. On the Leica SP5, we do not seem to be able to do this, because apparently only one data window can be opened, and if a new scan is done, it replaces the old data in that window.
I suppose I can use ImageJ to open the stored data, but i was wondering if we are missing something obvious.
--aryeh
--
Aryeh Weiss
School of Engineering
Bar Ilan University
Ramat Gan 52900 Israel
Ph: 972-3-5317638
FAX: 972-3-7384050
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Zoltan Cseresnyes
Facility manager, Imaging Suite
University of Cambridge, UK