Re: Leica SP5 question

Posted by Aryeh Weiss on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-25-X-Plan-Neofluor-Phase-3-objective-for-sale-tp2286535p2301077.html

First -- thanks for the quick responses. We will ask our rep to get us the
software upgrade (the system is only a few months old -- I assume that getting
the software will not be a problem).

Concerning mark and find with live data mode,
I think that it is not available on our machine. This is a problem because we
have not been able to find a way to do a multipoint timelapse acquisition.

Maybe the experienced SP5 users can correct me on that one. Is there a way to
set up an XYZT acquisition with multiple fields?

--aryeh

Adrian Smith wrote:

> Also, does the Mark & Find function work in Live Data in version 2?
>
> When I had a quick look at in 1.8.2 it  didn't seem like "mark and find"
> was available in Live Data mode? (or at least I couldn't see it).
>
> Regards,
>
> Adrian Smith
> Centenary Institute, Sydney, Australia
>  
>
>
> On 10/02/2009, at 2:32 PM, Dr. Graham Wright wrote:
>
>> Hello Zoltan & listers,
>>  
>> You comments suggest that you are a regular user of the Leica Live
>> Data Mode on the SP5... if so, I was wondering how you handle the data
>> post-acquisition - to get back to individual time series from each of
>> the different positions. We tend to use ImageJ and macros we have
>> written to sort and concatenate the files, but I am interested in how
>> others approach this.
>>  
>> Best wishes,
>> Graham
>>
>>     ----- Original Message -----
>>     *From:* Zoltan Cseresnyes <mailto:[hidden email]>
>>     *To:* [hidden email]
>>     <mailto:[hidden email]>
>>     *Sent:* Tuesday, February 10, 2009 6:36 AM
>>     *Subject:* Re: Leica SP5 question
>>
>>     Aryeh,
>>
>>     If you upgrade to LAS AF version 2.0, you'll be able to open a
>>     second image window and observe previously acquired data. We very
>>     much like the new version anyway, e.g. we find the Mark and Find
>>     and Live Data Mode more stable than even with the 1.8.2, which was
>>     already pretty good.
>>       I hope this helps,
>>
>>     Zoltan
>>
>>
>>
>>     On Mon, Feb 9, 2009 at 10:31 PM, Aryeh Weiss <[hidden email]
>>     <mailto:[hidden email]>> wrote:
>>
>>         When acquiring confocal data, we find it useful to have a
>>         previous experiment (usually an image acquired earlier during
>>         that session) open, while making the live acquisition. This
>>         allows an immediate visual comparison of the current result
>>         with a previous result. On the Leica SP5, we do not seem to be
>>         able to do this, because apparently only one data window can
>>         be opened, and if a new scan is done, it replaces the old data
>>         in that window.
>>
>>         I suppose I can use ImageJ to open the stored data, but i was
>>         wondering if we are missing something obvious.
>>
>>         --aryeh
>>         --
>>         Aryeh Weiss
>>         School of Engineering
>>         Bar Ilan University
>>         Ramat Gan 52900 Israel
>>
>>         Ph:  972-3-5317638
>>         FAX: [phone]
>>
>>
>>
>>
>>     --
>>
>>     Zoltan Cseresnyes
>>     Facility manager, Imaging Suite
>>     University of Cambridge, UK
>