Re: Leica SP5 question

Posted by Caterina Strambio on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-25-X-Plan-Neofluor-Phase-3-objective-for-sale-tp2286535p2301785.html

There you go!

Caterina


____________________________
Caterina Strambio De Castillia, Dottore in Biologia, PhD
Senior Research Scientist
Laboratory of Viral replication, pathogenesis, and immunity

http://www.mimo.unige.ch/luban_lab/

Department of Microbiology and Molecular Medicine
University of Geneva
Switzerland

Phone: +41 (0)78 7367537

****
Visiting Scientist
Laboratory of Cellular and Structural Biology
The Rockefeller University
1230 York Avenue
New York NY 10065



On 9-feb-09, at 23:36, Zoltan Cseresnyes wrote:

> Aryeh,
>
> If you upgrade to LAS AF version 2.0, you'll be able to open a  
> second image window and observe previously acquired data. We very  
> much like the new version anyway, e.g. we find the Mark and Find  
> and Live Data Mode more stable than even with the 1.8.2, which was  
> already pretty good.
>   I hope this helps,
>
> Zoltan
>
>
>
> On Mon, Feb 9, 2009 at 10:31 PM, Aryeh Weiss <[hidden email]>  
> wrote:
> When acquiring confocal data, we find it useful to have a previous  
> experiment (usually an image acquired earlier during that session)  
> open, while making the live acquisition. This allows an immediate  
> visual comparison of the current result with a previous result. On  
> the Leica SP5, we do not seem to be able to do this, because  
> apparently only one data window can be opened, and if a new scan is  
> done, it replaces the old data in that window.
>
> I suppose I can use ImageJ to open the stored data, but i was  
> wondering if we are missing something obvious.
>
> --aryeh
> --
> Aryeh Weiss
> School of Engineering
> Bar Ilan University
> Ramat Gan 52900 Israel
>
> Ph:  972-3-5317638
> FAX: 972-3-7384050
>
>
>
> --
>
> Zoltan Cseresnyes
> Facility manager, Imaging Suite
> University of Cambridge, UK