Posted by
Pascal Lorentz-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Configuration-problem-with-Zeiss-LSM-510-tp2303218p2303403.html
Hi Yi,
this is basically not a problem, it's normal behavior.
You have to adjust the pinhole for each configuration. As Esteban
already said this can be done in the Maintain > Pinhole menu. You have
to center the pinhole in front of the detector. In most of the cases you
will anyway get an image even if the pinhole is not adjusted but it
might be of uneven brightness.
Cheers
Pascal
--
Pascal Lorentz
Department of Biomedicine
University of Basel
Mattenstrasse 28
4058 Basel
Switzerland
yee z schrieb:
> Dear listers,
>
> We have a Zeiss LSM510 confocal microscopy system at our core
> facility. And I got some problems while writing new configuration.
> Here is an example:
> Single track configuartion for FITC, I used to use HFT
> 405/488/543/633, NFT 545, NFT 490 and BP505-550. It works fine.
> (Screen shot:
http://i561.photobucket.com/albums/ss60/mizhzmkm/Q-1A.jpg)
>
> But when I try to change the primary dichroic beam splitter to HFT
> 488/594 and keep everything else the same, the systme shows "Pinhole
> PH3 is not adjusted". (Screen shot:
>
http://i561.photobucket.com/albums/ss60/mizhzmkm/Q-1B.jpg)
>
> Also if I change the NFT 545 to NFT 595, the system also shows
> "Pinhole PH3 is not adjusted" (Screen shot:
>
http://i561.photobucket.com/albums/ss60/mizhzmkm/Q-1C.jpg)
> I am still be able to take the images regardless the error window.
>
> So does anyone here have the same problem? What does "Pinhole PH3 is
> not adjusted" mean, any reason for this?
>
> Any suggestion will be appreciated.
>
> Thanks,
>
> Yi
>
>
>