Re: Zeiss SD

Posted by Fred Mast on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-SD-tp2303456p2304344.html

Does the CSU-X1 allow you to change the disks? I'm still waiting for a spinning-disk unit that is actually designed for live-cell imaging. The previous Yokogawa units were optimized for 100x 1,4 NA oil objectives if I'm not mistaken. Even with relatively thin samples (cultured cells), you're still introducing spherical aberration...

By comparison, has anyone else looked at the technology being developed in Raphael Yuste's laboratory? They have combined multiphoton LSM with spatial light modulation technology to effectively "scan" an entire field at once. I'm not an expert in this but in my opinion if you could make it fast enough this seems like a better option to me (they report 60Hz calcium ion imaging). You can check it out here (under the SLM Microscopy section):

http://www.columbia.edu/cu/biology/faculty/yuste/methods.html

Cheers,

Fred

On 10-Feb-09, at 10:55 AM, G. Esteban Fernandez wrote:

I too was intrigued when I saw Zeiss roll out a spinning disk.  We're happy with our 5 LIVE.  Being able to vary the slit aperture size to match different objectives/samples is important in our multi-user facility.  It's also nice to capture two fluorescent channels simultaneously.  I wish the detector on the 5 LIVE were EM-CCD but the regular CCD does the job.  Images sometimes have vertical line artifacts but those are nicely dealt with by Fourier filtering or dividing by a blank image.  Must be lower cost (if that's true) and/or established familiarity of the spinning disk that makes them easy to sell.

 

-Esteban

On Tue, Feb 10, 2009 at 10:35 AM, Simon Walker <[hidden email]> wrote:

I was interested to see that Zeiss have gone into partnership with Yokogawa
to launch their own flavour of the spinning disk confocal:
http://www.microscopy-analysis.com/news/carl-zeiss-and-yokogawa-launch-
spinning-disk-cell-observer-sd-2008

Does this represent an acknowledgment that the 5-live doesn't do everything
it says on the tin, or just that it's too expensive?  Are there any happy 5-live
users out there?

--
G. Esteban Fernandez, Ph.D.
Associate Director
Molecular Cytology Core Facility
University of Missouri
120 Bond Life Sciences Center
Columbia, MO  65211

http://www.biotech.missouri.edu/mcc/

573-882-4895
573-884-9395 fax

Fred D. Mast

Department of Cell Biology

Medical Sciences Building Room 5-14

University of Alberta

Edmonton, Alberta, T6G 2H7

Canada

Tel: [phone]

[hidden email]