Re: Leica SP5 question - Motorised Stage

Posted by Adrian Smith-6 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-25-X-Plan-Neofluor-Phase-3-objective-for-sale-tp2286535p2305659.html

On 10/02/2009, at 4:59 PM, Cameron Nowell wrote:

>  To be able to do XYZT acquisition with multiple fields your
> scope needs to be fitted with a motorised stage. If you have that then
> you will be fine.


While we are talking SP5 and motorised stage....

I've been wondering if anyone has a come up with a good procedure for  
setting up the calibration of the stage position, ie in the Stage  
Configuration window there are settings for Tile Scan overlap, origin  
offset sizes, merge settings that need to be set up in order for tile  
scans to work.

One of the students here got them quite close through a laborious  
trial-and-error process. Unfortunately, we need to reset them because  
the stage alignment got messed up at one point (when the stage cable  
got caught in the opening into the incubation enclosure).

Before I go through the process of doing it by trial and error or  
working out a more systematic way of doing it I was wondering if  
anyone else had any suggestions as to how best approach it?

Regards,

Adrian Smith
Centenary Institute, Sydney, Australia