Re: Leica SP5 question
Posted by
Zoltan on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-25-X-Plan-Neofluor-Phase-3-objective-for-sale-tp2286535p2305865.html
Hi Graham,
We do it similarly, except that I wrote a C# software toolkit that separates the Leica files into folders based on various criteria, taking advantage of Leica's fairly consistent file-naming structure. Bit of work that extends post-processing time but we learned to live with it.
Best,
Zoltan
On Tue, Feb 10, 2009 at 3:32 AM, Dr. Graham Wright
<[hidden email]> wrote:
Hello Zoltan & listers,
You comments suggest that you are a regular user of
the Leica Live Data Mode on the SP5... if so, I was wondering how you handle the
data post-acquisition - to get back to individual time series from each of
the different positions. We tend to use ImageJ and macros we have written
to sort and concatenate the files, but I am interested in how others approach
this.
Best wishes,
Graham
----- Original Message -----
Sent: Tuesday, February 10, 2009 6:36
AM
Subject: Re: Leica SP5 question
Aryeh,
If you upgrade to LAS AF version
2.0, you'll be able to open a second image window and observe previously
acquired data. We very much like the new version anyway, e.g. we find the Mark
and Find and Live Data Mode more stable than even with the 1.8.2, which was
already pretty good.
I hope this helps,
Zoltan
On Mon, Feb 9, 2009 at 10:31 PM, Aryeh Weiss
<[hidden email]>
wrote:
When
acquiring confocal data, we find it useful to have a previous experiment
(usually an image acquired earlier during that session) open, while making
the live acquisition. This allows an immediate visual comparison of the
current result with a previous result. On the Leica SP5, we do not seem to
be able to do this, because apparently only one data window can be opened,
and if a new scan is done, it replaces the old data in that window.
I
suppose I can use ImageJ to open the stored data, but i was wondering if we
are missing something obvious.
--aryeh
--
Aryeh Weiss
School of Engineering
Bar Ilan University
Ramat Gan
52900 Israel
Ph: 972-3-5317638
FAX:
972-3-7384050
--
Zoltan Cseresnyes
Facility manager, Imaging Suite
University of Cambridge, UK
--
Zoltan Cseresnyes
Facility manager, Imaging Suite
University of Cambridge, UK