Spinning disk alignment

Posted by Knecht, David on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Spinning-disk-alignment-tp2306574.html

I am wondering if anyone out there with a Yokogawa spinning disk system has attempted to optimize the alignment/positioning of the various components and worked out a good way to do it.  We have recently installed a system which has in order a Nikon TI,  Yokogawa CSU-X, Sutter filter wheel and camera.  How critical is the positioning  of the Yokogawa relative to the microscope?  How do you optimize that?  The position of the camera relative to the CSU seems simple in that you would optimize for a sharp image on the camera.  What is a good sample to do this with? Any hints appreciated.   Thanks- Dave

Dr. David Knecht    

Department of Molecular and Cell Biology

Co-head Flow Cytometry and Confocal Microscopy Facility

U-3125

91 N. Eagleville Rd.

University of Connecticut

Storrs, CT 06269

860-486-2200

860-486-4331 (fax)