Re: Spinning disk alignment

Posted by Robin Battye on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Spinning-disk-alignment-tp2306574p2308292.html

Hi Dr. Knecht,

    Please feel free to contact me off line and I will be happy to tell you what I can.

Cheers Robin


On 10/02/09 10:04 PM, "David Knecht ATT" <david.knecht@...> wrote:

I am wondering if anyone out there with a Yokogawa spinning disk system has attempted to optimize the alignment/positioning of the various components and worked out a good way to do it.  We have recently installed a system which has in order a Nikon TI,  Yokogawa CSU-X, Sutter filter wheel and camera.  How critical is the positioning  of the Yokogawa relative to the microscope?  How do you optimize that?  The position of the camera relative to the CSU seems simple in that you would optimize for a sharp image on the camera.  What is a good sample to do this with? Any hints appreciated.   Thanks- Dave

 
Dr. David Knecht    
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)

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