Re: Leica SP5 question

Posted by George Ring on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-25-X-Plan-Neofluor-Phase-3-objective-for-sale-tp2286535p2312636.html

Hi, Zoltan,

The Mark and Find function apparently only works in TCS SP5 mode in 1.8.2.
In TCS SP5 mode, the Tile Scan and Mark and Find function buttons are
active, but when I switched to Live Data Mode they became greyed
out/inactive.  It seems to be the nature of the beast.

George


George Ring, Ph.D.
Dept. of Cell and Developmental Biology
SUNY Upstate Medical University
750 E. Adams St.
Syracuse NY  13210
Tel. [phone]
FAX [phone]
email: [hidden email]



>>> Zoltan Cseresnyes <[hidden email]> 02/11/09 6:21 PM >>>
Adrian,
The Mark and Find still doesn't work in the Live Data Mode, even in version
2.

Zoltan


On Tue, Feb 10, 2009 at 3:55 AM, Adrian Smith <[hidden email]
> wrote:

> Also, does the Mark & Find function work in Live Data in version 2?
>
> When I had a quick look at in 1.8.2 it  didn't seem like "mark and find"
> was available in Live Data mode? (or at least I couldn't see it).
>
> Regards,
>
> Adrian Smith
> Centenary Institute, Sydney, Australia
>
>
>
> On 10/02/2009, at 2:32 PM, Dr. Graham Wright wrote:
>
> Hello Zoltan & listers,
>
> You comments suggest that you are a regular user of the Leica Live Data
> Mode on the SP5... if so, I was wondering how you handle the data
> post-acquisition - to get back to individual time series from each of the
> different positions. We tend to use ImageJ and macros we have written to
> sort and concatenate the files, but I am interested in how others approach
> this.
>
> Best wishes,
> Graham
>
> ----- Original Message -----
> *From:* Zoltan Cseresnyes <[hidden email]>
> *To:* [hidden email]
> *Sent:* Tuesday, February 10, 2009 6:36 AM
> *Subject:* Re: Leica SP5 question
>
> Aryeh,
>
> If you upgrade to LAS AF version 2.0, you'll be able to open a second

image

> window and observe previously acquired data. We very much like the new
> version anyway, e.g. we find the Mark and Find and Live Data Mode more
> stable than even with the 1.8.2, which was already pretty good.  I hope
> this helps,
>
> Zoltan
>
>
>
> On Mon, Feb 9, 2009 at 10:31 PM, Aryeh Weiss <[hidden email]> wrote:
>
>> When acquiring confocal data, we find it useful to have a previous
>> experiment (usually an image acquired earlier during that session) open,
>> while making the live acquisition. This allows an immediate visual
>> comparison of the current result with a previous result. On the Leica

SP5,
>> we do not seem to be able to do this, because apparently only one data
>> window can be opened, and if a new scan is done, it replaces the old data
in
>> that window.
>>
>> I suppose I can use ImageJ to open the stored data, but i was wondering
if

>> we are missing something obvious.
>>
>> --aryeh
>> --
>> Aryeh Weiss
>> School of Engineering
>> Bar Ilan University
>> Ramat Gan 52900 Israel
>>
>> Ph:  972-3-5317638
>> FAX: [phone]
>>
>
>
>
> --
>
> Zoltan Cseresnyes
> Facility manager, Imaging Suite
> University of Cambridge, UK
>
>
>

--

Zoltan Cseresnyes
Facility manager, Imaging Suite