Confocal Microscopy List

Leica DMI6000 perfusion system

Posted by Paloma Domínguez Giménez on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-LSM710-tp2337651p2340737.html

Dear all,

I write you because I am trying to install a perfusion system in an inverted Leica DMI6000 microscope and I was wondering if any of you had the same problems before.

Our microscope is installed in an incubator ML and it is equipped also with a heating insert P (heating stage).

This stage is a solid rectangular piece made in aluminium and is designed with a couple of openings (one in front the other) for the tubes that will allow the medium exchange.

BUT, the system does not come with a pump or the tubes for the perfusion.

I contacted Leica to check whether I was missing any parts of the system, but they suggested me I have to install them.

I checked then if the perfusion system FCS2 was suitable for DMI6000 microscopes, and the FCS chamber is for a circular stage, so I can not adapt that to my stage….

I was wondering if the solution is to buy a pump and the tubes independently.

Does anyone have any idea on how to solve this?

Thanks a lot in advanced!!!!

Paloma Dominguez, PhD.

Microscopy facility

Andalusian Center of Molecular Biology and Regenerative Medicine-CABIMER

Parque Tecnologico Cartuja 93

Avda. Americo Vespucio s/n

41092 Sevilla

Spain

Tel.:+34 954 46 8223

Fax:+34 954 46 1664