Re: Leica DMI6000 perfusion system
Posted by
Julio Vazquez on
Feb 17, 2009; 5:36pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-LSM710-tp2337651p2342161.html
Hi Paloma,
What are your samples? are they just in a Petri dish inside your heating insert P? Can you heat the whole incubator, or just the insert?
I can only give you some general pointers. Bioptechs has pumps and other accessories for perfusion. You may be able to build your own system from their components (pump, tubing, and needles), maybe with a bit of help from your mechanical shop:
If that's too difficult, you could get one of their open dish systems, such as Delta T, where there are already holes for the accessories and such. If your incubator is heatable, all you need are the dishes and stage insert. They should have them for most microscope stage fittings. If you can't heat the incubator, then you would need also the heating control unit... obviously, this would add a few thousand Euros. For more info, check their website and/or catalog:
I have no commercial interest with Bioptechs... just familiar with their products. Other vendors of microscope accessories should have similar products. I am surprised Leica wouldn't be able to help you with that. One European supplier of accessories for Leica and Zeiss microscopes is Pe-Con:
They do have some perfusion accessories which should fit Zeiss or Leica stage inserts (your stage insert P is probably from them).
Buena suerte,
Julio.
--
Julio Vazquez
Fred Hutchinson Cancer Research Center
Seattle, WA 98109-1024
On Feb 17, 2009, at 4:28 AM, Paloma Domínguez Giménez wrote:
Dear all,
I write you because I am trying to install a perfusion system in an inverted Leica DMI6000 microscope and I was wondering if any of you had the same problems before.
Our microscope is installed in an incubator ML and it is equipped also with a heating insert P (heating stage).
This stage is a solid rectangular piece made in aluminium and is designed with a couple of openings (one in front the other) for the tubes that will allow the medium exchange.
BUT, the system does not come with a pump or the tubes for the perfusion.
I contacted Leica to check whether I was missing any parts of the system, but they suggested me I have to install them.
I checked then if the perfusion system FCS2 was suitable for DMI6000 microscopes, and the FCS chamber is for a circular stage, so I can not adapt that to my stage….
I was wondering if the solution is to buy a pump and the tubes independently.
Does anyone have any idea on how to solve this?
Thanks a lot in advanced!!!!
Paloma Dominguez, PhD.
Microscopy facility
Andalusian Center of Molecular Biology and Regenerative Medicine-CABIMER
Parque Tecnologico Cartuja 93
Avda. Americo Vespucio s/n
41092 Sevilla
Spain
Tel.:+34 954 46 8223
Fax:+34 954 46 1664