Confocal Microscopy List

Re: Green HeNe543nm laser

Posted by Guy Cox on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Green-HeNe543nm-laser-tp2351352p2352457.html

Ghafar,

The Radiance uses a polarizing beam-splitter so it is essential to use a linear polarised laser (and to orient it correctly).

Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
    http://www.guycox.com/optical.htm
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Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
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Phone +61 2 9351 3176     Fax +61 2 9351 7682
Mobile 0413 281 861
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     http://www.guycox.net

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ghafar Sarvestani
Sent: Thursday, 19 February 2009 4:29 PM
To: [hidden email]
Subject: Green HeNe543nm laser

Dear All,

I am going to replace my Green HeNe 543nm laser tube in our Bio-Rad 2100 radiance system. I need your input regarding the choice of the Green HeNe laser543nm.

1. Should I order the linearly polarized configuration or randomly polarized Green HeNe 543nm? Which one is suitable for Radiance confocal microscope?

2.What should be the minimum output?

Thank you very much in advance.

Regards,

Ghafar

***********************************************

Ghafar T Sarvestani, PhD

Hanson Institute for Medical Research

Hanson Building level 2, Room 2-42-N

IMVS, Frome Road, Adelaide SA 5000

Affiliate Lecturer

Department of Medicine

The University Of Adelaide

Tel: +61 8 82223603

Fax: +61 8 82223162

Email:[hidden email]

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