Resolution for fluorescence, brightfield and luminescence

Posted by Monique Vasseur on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Live-cell-Imaging-with-fluoview-1000-tp2242987p2353750.html

Dear all,

My question is:
On the same microscope, same objective, same immersion medium and same
sample, is there a difference in resolution depending of the microscopy
method I am using (fluorescence, luminescence or brightfield) since the
lightpath is not the same?

Is it correct to consider the following?

For brightfield:  r = (1.22 * illumination wavelenght)/(NA objective +
NA condenser)
For fluorescence and confocal: r = (0.61 * excitation (?) wavelenght) /
NA objective
For luminescence: r = (0.61 * emission (?) wavelenght) / NA objective

Thanks in advance,

monique