Re: Resolution for fluorescence, brightfield and luminescence

Posted by Arwed Weigel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Live-cell-Imaging-with-fluoview-1000-tp2242987p2358353.html

All these formulas for confocal microscope resolution assume a
homogeneously illuminated back-aperture, which is not always the case in
commercially available microscopes due to the Gaussian intensity profile
of the excitation laser beam. Therefore, the lateral resolution of a
confocal microscope can even be worse than of a widefield fluorescence
microscope with arc-lamp illumination.

--
arwed weigel
abteilung fuer neurophysiology und zellulaere biophysik
universitaet goettingen
humboldtallee 23
37073 goettingen

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