Posted by
Andreas Bruckbauer on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Zeiss-LSM710-tp2337651p2374291.html
I guess that depends on your sample and laser power, during our demo they
imaged right over the laser lines and you could see nothing of the laser,
but this was with one of the usual super bright test samples. In this case
the laser lines are completely filtered out by the dichroics.
I later did some test on individual, multiple fluorophore labelled Fab's
(cy3) attached directly to a coverslip, you loose about 3 images (30 nm), i
had to open the pinhole a bit to get good S/N this might have affected the
laser line suppression. However the signal was impressive for a confocal.
According to Zeiss you can get the LSM 710 with special dichroics for single
molecule detection (FCS) which have double the suppression than the ususal
ones, but this is more expensive.
Andreas
On Mon, 23 Feb 2009 10:32:30 +0200, Aryeh Weiss <
[hidden email]> wrote:
>Have people with the LSM710 checked how close the laser lines the system can
>image? If so, I would be very interested in the results.
>
>--aryeh
>--
>Aryeh Weiss
>School of Engineering
>Bar Ilan University
>Ramat Gan 52900 Israel
>
>Ph: 972-3-5317638
>FAX: 972-3-7384050
>
>Israel Society for Microscopy 2009 meeting website:
>
http://materials.technion.ac.il/ism/ISM2009.html>
>Khaled Machaca wrote:
>> Claire,
>>
>> We've purchased one and have been using it for a few weeks. It is a
>> significant upgrade from the 510. More sensitive and the spectral
>> imaging is wonderful.
>>
>> -Khaled
>>
>>
>>
>> On Feb 17, 2009, at 1:14 AM, Claire Brown, Dr. wrote:
>>
>>> I was wondering if people have one or have done extensive demos on the
>>> new Zeiss LSM710. I would be interested in general feedback about the
>>> system and its performance. I am particularly interested in
>>> sensitivity and the new spectral detectors.
>>>
>>> Feel free to contact me offline if you like.
>>>
>>> Sincerely,
>>>
>>> Claire
>>>
>>> ****NOTE MY NEW ADDRESS****
>>> Claire M. Brown, PhD
>>> Life Sciences Complex Imaging Facility Director
>>> McGill University Department of Biochemistry
>>> Bellini Building, Rm 137A
>>> 3649 Promenade Sir William Osler
>>> Montreal, Quebec, H3G 0B1
>>> 514-398-4400 ext 00795 (Phone)
>>> 514-398-7452 (FAX)
>>> /
[hidden email]/ <mailto:
[hidden email]>
>>> */
http://www.lifesciencescomplex.mcgill.ca/imaging/*>>>
>>