Re: poor dapi images on LSM 510

Posted by Julian Smith III on
URL: http://confocal-microscopy-list.275.s1.nabble.com/poor-dapi-images-on-LSM-510-tp2398732p2398849.html

Sudden death of the 405?  We lost ours at 560+/- user hours (Olympus FV1000; laser replaced under warranty).  New 405 is running at 5% on the same preps.
JSIII

-----Original Message-----
From: Confocal Microscopy List on behalf of Carl Boswell
Sent: Fri 2/27/2009 6:19 PM
To: [hidden email]
Subject: poor dapi images on LSM 510
 
We have been having a chronic problem with the image quality (S/N,
resolution) of DAPI stained nuclei using a Zeiss 510 Meta.  The quality
seems to decay over time (months) and pinhole/collimator tuning doesn't make
things right.  We have had the AOM replaced once but the problem eventually
returned.  We now have to use 100% 405 laser, rather than 10% to get
anything close to good images.  Is there something about the AOM stability
in this light path, or alignment issues with the laser that others have
found to result in a slow decay in usefulness of this channel?

Thanks for your thoughts.
Carl

Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-954-7053
FAX [phone]