Re: poor dapi images on LSM 510
Posted by
O'Toole, P on
URL: http://confocal-microscopy-list.275.s1.nabble.com/poor-dapi-images-on-LSM-510-tp2398732p2400856.html
You may also want to check that the optic fibre is not being knocked. The
405 output on the 510 is sensitive to the optic fibre being moved close to
the back of the laser module. This could account for the decay over time if
the laser output remains high.
Best
Pete
----- Original Message -----
From: "Carl Boswell" <
[hidden email]>
To: <
[hidden email]>
Sent: Friday, February 27, 2009 11:19 PM
Subject: poor dapi images on LSM 510
> We have been having a chronic problem with the image quality (S/N,
> resolution) of DAPI stained nuclei using a Zeiss 510 Meta. The quality
> seems to decay over time (months) and pinhole/collimator tuning doesn't
> make things right. We have had the AOM replaced once but the problem
> eventually returned. We now have to use 100% 405 laser, rather than 10%
> to get anything close to good images. Is there something about the AOM
> stability in this light path, or alignment issues with the laser that
> others have found to result in a slow decay in usefulness of this channel?
>
> Thanks for your thoughts.
> Carl
>
> Carl A. Boswell, Ph.D.
> Molecular and Cellular Biology
> University of Arizona
> 520-954-7053
> FAX 520-621-3709