GFP/RFP quenching

Posted by Scott Howell-3 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/GFP-RFP-quenching-tp2422621.html

List,

We have had some issues here with a lab where the GFP/RFP brightness in
fixed cells has become extremely variable. Like night and day. Wondering
if it may be related to the pH of their paraformaldehyde fix? Does one
particular pH seem to work best?? Any other ideas? Thanks.

Scott J. Howell, Ph.D.
Manager, Imaging Module
Visual Sciences Research Center
Case Western Reserve University
2085 Adelbert Rd.
Institute of Pathology Room 106
Cleveland, Ohio 44106
216-368-2300
http://www.case.edu/med/vsrc/