Posted by
Guy Cox on
URL: http://confocal-microscopy-list.275.s1.nabble.com/question-about-laser-tp2424346p2427544.html
Picoquant supply diode lasers (pulsed or cw) from 375nm
to something like 470nm (you can get whatever you want.)
I have no connection with the company (except as a
satisfied customer). I'm sure there are other suppliers
as well. They are quite cheap (a tiny fraction of a
frequency doubled TiS). You do have to think a bit about
your optics at this wavelength (and how you align a beam
you can't see).
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
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Phone +61 2 9351 3176 Fax +61 2 9351 7682
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-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]]
On Behalf Of
[hidden email]
Sent: Thursday, 5 March 2009 10:48 AM
To:
[hidden email]
Subject: Re: question about laser
Sorry for the confusion. I wasn't clear enough about my question. You
are right, Craig. I'm looking for a laser line that's between 380nm
and 400nm.
Thank you all for the good suggestions!
Yue
Quoting Mario <
[hidden email]>:
> Speaking of which, whatever happened to the promised solid state laser
> that is basically the same as the 405 nm but tuned to emit as short as
> 385 nm? I may be a bit off, but the DAPI fluorescence emission is
about
> 7 times higher at 385 nm (~45% max) compared to 405 nm (~6% max).
>
> Mario
>
>> Ah, just realized I misunderstood your question. You just need a
>> laser line between 380 and 400. Your best bet there would be to
>> get a high-powered IR diode laser and use a crystal to double it.
>> A diode laser at 780nm doubled would give you 390nm.
>>
>> Craig
>>
>> On Wed, Mar 4, 2009 at 11:49 AM, Craig Brideau
>> <<mailto:
[hidden email]>
[hidden email]> wrote:
>>
>> A CW laser is by definition narrowband; there's no way you can have
>> one with 20nm spectral bandwidth. The only thing I can think of
>> off hand that would give you the spectral range you are looking for
>> is an LED. The only other alternative would be something like a
>> supercontinuum (or so-called 'white' laser) but that emits over a
>> huge range and would be overkill. I'd recommend trying to find a
>> really powerful LED in that range, or even a bunch of lower-powered
>> LEDs bundled into an array.
>>
>> Craig
>>
>>
>>
>> On Wed, Mar 4, 2009 at 10:43 AM,
>> <<mailto:
[hidden email]>
[hidden email]> wrote:
>>
>> Hi everyone,
>>
>> We are looking for a coherent (or continuous wave) laser ranging
>> from 380nm to 400nm. So far we haven't found anything impressive.
>> Does anyone know any laser between that range? Thanks a lot!
>>
>> Yue
>
>
> --
>
________________________________________________________________________
________
> Mario M. Moronne, Ph.D.
>
>
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>
[hidden email]
>
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