Re: question about laser

Posted by yue_li on
URL: http://confocal-microscopy-list.275.s1.nabble.com/question-about-laser-tp2424346p2430670.html

Thank you so much for the information. Cost is a big factor in our  
decision. I'll check that company out. We can't align the laser in the  
lab, but we have someone else help us to do it.

Yue


Quoting Guy Cox <[hidden email]>:

> Picoquant supply diode lasers (pulsed or cw) from 375nm
> to something like 470nm (you can get whatever you want.)
> I have no  connection with the company (except as a
> satisfied customer).  I'm sure there are other suppliers
> as well.  They are quite cheap (a tiny fraction of a
> frequency doubled TiS).  You do have to think a bit about
> your optics at this wavelength (and how you align a beam
> you can't see).
>
>                                              Guy
>
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox    CRC Press / Taylor & Francis
>    http://www.guycox.com/optical.htm
> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Electron Microscope Unit, Madsen Building F09,
> University of Sydney, NSW 2006
> ______________________________________________
> Phone [phone]     Fax [phone]
> Mobile [phone]       http://www.guycox.net
> ______________________________________________
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of [hidden email]
> Sent: Thursday, 5 March 2009 10:48 AM
> To: [hidden email]
> Subject: Re: question about laser
>
> Sorry for the confusion. I wasn't clear enough about my question. You
> are right, Craig. I'm looking for a laser line that's between 380nm
> and 400nm.
>
> Thank you all for the good suggestions!
>
> Yue
>
>
> Quoting Mario <[hidden email]>:
>
>> Speaking of which, whatever happened to the promised solid state laser
>> that is basically the same as the 405 nm but tuned to emit as short as
>> 385 nm? I may be a bit off, but the DAPI fluorescence emission is
> about
>> 7 times higher at 385 nm (~45% max) compared to 405 nm (~6% max).
>>
>> Mario
>>
>>> Ah, just realized I misunderstood your question.  You just need a
>>> laser line between 380 and 400.  Your best bet there would be to
>>> get a high-powered IR diode laser and use a crystal to double it.
>>> A diode laser at 780nm doubled would give you 390nm.
>>>
>>> Craig
>>>
>>> On Wed, Mar 4, 2009 at 11:49 AM, Craig Brideau
>>> <<mailto:[hidden email]>[hidden email]> wrote:
>>>
>>> A CW laser is by definition narrowband; there's no way you can have
>>>  one with 20nm spectral bandwidth.  The only thing I can think of
>>> off hand that would give you the spectral range you are looking for
>>>  is an LED.  The only other alternative would be something like a
>>> supercontinuum (or so-called 'white' laser) but that emits over a
>>> huge range and would be overkill.  I'd recommend trying to find a
>>> really powerful LED in that range, or even a bunch of lower-powered
>>>  LEDs bundled into an array.
>>>
>>> Craig
>>>
>>>
>>>
>>> On Wed, Mar 4, 2009 at 10:43 AM,
>>> <<mailto:[hidden email]>[hidden email]> wrote:
>>>
>>> Hi everyone,
>>>
>>> We are looking for a coherent (or continuous wave) laser ranging
>>> from 380nm to 400nm. So far we haven't found anything impressive.
>>> Does anyone know any laser between that range? Thanks a lot!
>>>
>>> Yue
>>
>>
>> --
>>
> ________________________________________________________________________
> ________
>> Mario M. Moronne, Ph.D.
>>
>> [hidden email]
>> [hidden email]
>> [hidden email]
>
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