Re: question about laser

Posted by Guy Cox on
URL: http://confocal-microscopy-list.275.s1.nabble.com/question-about-laser-tp2424346p2433948.html

Can't you just check your fluorochrome on a spectrofluorimeter?

If for some reason you actually need to form images, why not
use a tunable filter with a mercury or metal halide lamp?
(Or even just buy a few different excitation filters if a
tunable filter is too dear.)

                                              Guy



Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
    http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
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-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of [hidden email]
Sent: Friday, 6 March 2009 2:48 PM
To: [hidden email]
Subject: Re: question about laser

I just want one fixed wavelength within that range eventually. The problem is that I don't know which one will work the best on my sample. I only know it should lay between 380 and 400nm from my other experiments. And I'm hoping to find an inexpensive way to find out.

Yue


Quoting Guy Cox <[hidden email]>:

> Yes, he was referring to a doubler.  The problem is people are still
> not clear whether you want to tune the wavelength between 380 and 400
> nm  (in which case Sergey's suggestion is the most practical way to
> go, but is very expensive) or whether you just want one fixed
> wavelength within that range, which is easy and cheap to get with a
> diode.
>
>                                                   Guy
>
>
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox    CRC Press / Taylor & Francis
>     http://www.guycox.com/optical.htm
> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope Unit,
> Madsen Building F09, University of Sydney, NSW 2006
> ______________________________________________
> Phone [phone]     Fax [phone]
> Mobile [phone]
> ______________________________________________
>      http://www.guycox.net
> -----Original Message-----
> From: Confocal Microscopy List  
> [mailto:[hidden email]] On Behalf Of  
> [hidden email]
> Sent: Friday, 6 March 2009 4:18 AM
> To: [hidden email]
> Subject: Re: question about laser
>
> I don't quite understand. The laser beam we are looking for is  
> between 380 and 400nm. Are you thinking of the doubler? Could you  
> please give more detail? Thanks a lot.
>
> Yue
>
>
> Quoting "Femto @Sciner" <[hidden email]>:
>
>> Second Harmonic of CW Ti:Sapphire tunable between 760 and 800 nm.
>>
>> Best regards,
>> Sergey Egorov
>> Del Mar Photonics
>> 4119 Twilight Ridge
>> San Diego, CA 92130
>> tel [phone]
>> fax [phone]
>> http://www.dmphotonics.com/
>>
>>
>> [hidden email] wrote:
>>> Hi everyone,
>>>
>>> We are looking for a coherent (or continuous wave) laser ranging
>>> from 380nm to 400nm. So far we haven't found anything impressive.
>>> Does anyone know any laser between that range? Thanks a lot!
>>>
>>> Yue
>>>
>
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