PFA fixing + triton, what happens to surface proteins ?

Posted by Jean-Pierre CLAMME-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/PFA-fixing-triton-what-happens-to-surface-proteins-tp2470205.html


Hi,

It might be a trivial question, but I was just wondering, if I fix cells  with PFA and thus cross-link surface proteins or other molecules on the surface of the cell, what would happen to them if I permeabilize the cell using 0.1% Triton? Will  they disappear with the membrane ? Will they stay cross-linked to some transmembranar proteins and thus with the inside of the cell ?

Any comments are welcome.

Thanks,

JP