Re: Immersion Oil

Posted by stu_the_flat on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Immersion-Oil-tp2491973p2492239.html

Isn't there a 0.002 difference in the diffraction between some of the immersion oils?

Off the top of my head

Olympus Oil = 1.515
Ziess Oil = 1.515
Nikon Oil = 1.513

I don’t think it make the world of difference but as I am interested in PSFs I always match the oil the microscope.

Stuart

David Knecht-charter wrote

Being a natural skeptic, that has been my presumption as well.  I  
haven't talked to anyone who has actually seen mixed oils gum up their  
scope or dissolve  lens cement (if you believe that last one, I have a  
few million dollars in my uncle's  account in Africa I will be happy  
to send you).  Dave

On Mar 17, 2009, at 11:37 AM, Peter Carroll wrote:

>
> > I have been told by a Nikon representative that Zeiss oil could  
> destroy the Nikon objective lens
>
> Of course you heard that... Nikon wants you to purchase /their/  
> consumables, not their competitors! Heh...

Dr. David Knecht
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)