Re: Immersion Oil

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Immersion-Oil-tp2491973p2492361.html

When you are down to the third decimal place it's not going to make a huge difference*.  You might want to try and see if you observe any difference between oil, but I suspect you won't see much change.

Craig

*unless you are talking about fiber optics or some optoelectronics...

On Tue, Mar 17, 2009 at 9:58 AM, stu_the_flat <[hidden email]> wrote:

Isn't there a 0.002 difference in the diffraction between some of the
immersion oils?

Off the top of my head

Olympus Oil = 1.515
Ziess Oil = 1.515
Nikon Oil = 1.513

I don’t think it make the world of difference but as I am interested in PSFs
I always match the oil the microscope.

Stuart

David Knecht-charter wrote:
>
> Being a natural skeptic, that has been my presumption as well.  I
> haven't talked to anyone who has actually seen mixed oils gum up their
> scope or dissolve  lens cement (if you believe that last one, I have a
> few million dollars in my uncle's  account in Africa I will be happy
> to send you).  Dave
>
> On Mar 17, 2009, at 11:37 AM, Peter Carroll wrote:
>
>>
>> > I have been told by a Nikon representative that Zeiss oil could
>> destroy the Nikon objective lens
>>
>> Of course you heard that... Nikon wants you to purchase /their/
>> consumables, not their competitors! Heh...
>
> Dr. David Knecht
> Department of Molecular and Cell Biology
> Co-head Flow Cytometry and Confocal Microscopy Facility
> U-3125
> 91 N. Eagleville Rd.
> University of Connecticut
> Storrs, CT 06269
> 860-486-2200
> 860-486-4331 (fax)
>
>
>
>

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