http://confocal-microscopy-list.275.s1.nabble.com/Immersion-Oil-tp2491973p2492607.html
from different vendors. I would do the experiment with multicolored
>
> It’s an easy test to prove that the last (third) decimal place makes a
> reasonable difference. Using the Cargille test kit (range of ~20 RI
> around 1.515 step by 0.001), you can acquired PSFs with the different
> oils and just take a look at the x,z projections. You’ll see a pretty
> dramatic shift in spherical aberration, even on small steps (ok, 0.001
> is pretty small change, but 0.002 change is measurable for sure). It
> gets even more interesting (dare I say fun) if you mount the beads in
> some low percentage agar so that you get beads at random distances
> from the coverglass! I did this one weekend and recommend it if you
> need a distraction (from grants for instance…).
>
> As for mixing oils, I always thought the big worry was that different
> brands might not be miscible and therefore you could introduce a new
> RI boundary, causing aberrations. I have never tested, or in fact seen
> this demonstrated (on purpose or otherwise). Nonetheless, I will
> continue to not mix my oils.
>
> Happy imaging,
>
> Paul
>
> Paul S. Maddox, PhD
> Assistant Professor
> Institute for Research in Immunology and Cancer
> Dept of Pathology and Cell Biol, U. de Montreal
> P.O. Box 6128, Station Centre-Ville
> Montréal QC H3C 3J7
> CANADA
>
> Courier:
> 2900, boulevard Édouard-Montpetit
> Pavillon Marcelle-Coutu, Quai 20
> Montreal QC H3T 1J4
> CANADA
>
>
[hidden email] <mailto:
[hidden email]>
> Ph: 514-343-7894
> Fax: 514-343-6843
>
> ------------------------------------------------------------------------
>
> *From:* Confocal Microscopy List
> [mailto:
[hidden email]] *On Behalf Of *Craig Brideau
> *Sent:* Tuesday, March 17, 2009 12:24 PM
> *To:*
[hidden email]
> *Subject:* Re: Immersion Oil
>
> When you are down to the third decimal place it's not going to make a
> huge difference*. You might want to try and see if you observe any
> difference between oil, but I suspect you won't see much change.
>
> Craig
>
> *unless you are talking about fiber optics or some optoelectronics...
>
> On Tue, Mar 17, 2009 at 9:58 AM, stu_the_flat <
[hidden email]
> <mailto:
[hidden email]>> wrote:
>
> Isn't there a 0.002 difference in the diffraction between some of the
> immersion oils?
>
> Off the top of my head
>
> Olympus Oil = 1.515
> Ziess Oil = 1.515
> Nikon Oil = 1.513
>
> I don’t think it make the world of difference but as I am interested
> in PSFs
> I always match the oil the microscope.
>
> Stuart
>
>
>
>
>
> David Knecht-charter wrote:
> >
> > Being a natural skeptic, that has been my presumption as well. I
> > haven't talked to anyone who has actually seen mixed oils gum up their
> > scope or dissolve lens cement (if you believe that last one, I have a
> > few million dollars in my uncle's account in Africa I will be happy
> > to send you). Dave
> >
> > On Mar 17, 2009, at 11:37 AM, Peter Carroll wrote:
> >
> >>
> >> > I have been told by a Nikon representative that Zeiss oil could
> >> destroy the Nikon objective lens
> >>
> >> Of course you heard that... Nikon wants you to purchase /their/
> >> consumables, not their competitors! Heh...
> >
> > Dr. David Knecht
> > Department of Molecular and Cell Biology
> > Co-head Flow Cytometry and Confocal Microscopy Facility
> > U-3125
> > 91 N. Eagleville Rd.
> > University of Connecticut
> > Storrs, CT 06269
> > 860-486-2200
> > 860-486-4331 (fax)
> >
> >
> >
> >
>
> --
> View this message in context:
>
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>