Re: Immersion Oil

Posted by Boswell, Carl A - (cboswell) on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Immersion-Oil-tp2491973p2492776.html

The stories are real, at least with respect to the miscibility of different oils.  While one might think that oil is oil, two different immersion oils together can behave like oil and water.  That is why I insist that samples first viewed on some other microscope be scrupulously cleaned of whatever oil was used before applying oil used with our Core systems. 

 

As for the issue of one manufacture's oil eating away another manufacture's lens, I'd consider the source of that information carefully.  I also use Cargille oil on multiple sytems and have no complaints.

C

 

Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-954-7053
FAX [phone]

----- Original Message -----

From: [hidden email]

To: [hidden email]

Sent: Tuesday, March 17, 2009 6:56 AM

Subject: Immersion Oil

We now have Nikon, Zeiss and Leica microscopes in our facility.  Is the problem of cross-contamination of immersion oil real or hypothetical?   Is it possible to use a single type of immersion oil for all, so that we don't have to worry about moving samples between microscopes?   Dave

Dr. David Knecht    

Department of Molecular and Cell Biology

Co-head Flow Cytometry and Confocal Microscopy Facility

U-3125

91 N. Eagleville Rd.

University of Connecticut

Storrs, CT 06269

860-486-2200

860-486-4331 (fax)