Posted by
Glen MacDonald-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Immersion-Oil-tp2491973p2493171.html
We also use Cargille on all of our microscopes and insist that
specimens are cleaned of other oils before viewing on our equipment.
Several years ago at the 3D of Microscopy of Living Cells course,
someone ran out of Nikon oil and borrowed some Zeiss oil. the
result was a thick mucous-like substance that took hours to clean from
the objective. Maybe the oils have been re-formulated since then and
behave differently. Mixing the likely suspects to observe the results
could be an interesting science projectt. But, as always, be careful
when exchanging fluids.
Regards,
Glen
On Mar 17, 2009, at 10:43 AM, Carl Boswell wrote:
> The stories are real, at least with respect to the miscibility of
> different oils. While one might think that oil is oil, two
> different immersion oils together can behave like oil and water.
> That is why I insist that samples first viewed on some other
> microscope be scrupulously cleaned of whatever oil was used before
> applying oil used with our Core systems.
>
> As for the issue of one manufacture's oil eating away another
> manufacture's lens, I'd consider the source of that information
> carefully. I also use Cargille oil on multiple sytems and have no
> complaints.
> C
>
> Carl A. Boswell, Ph.D.
> Molecular and Cellular Biology
> University of Arizona
> 520-954-7053
> FAX 520-621-3709
> ----- Original Message -----
> From: David Knecht
> To:
[hidden email]
> Sent: Tuesday, March 17, 2009 6:56 AM
> Subject: Immersion Oil
>
> We now have Nikon, Zeiss and Leica microscopes in our facility. Is
> the problem of cross-contamination of immersion oil real or
> hypothetical? Is it possible to use a single type of immersion oil
> for all, so that we don't have to worry about moving samples between
> microscopes? Dave
>
> Dr. David Knecht
> Department of Molecular and Cell Biology
> Co-head Flow Cytometry and Confocal Microscopy Facility
> U-3125
> 91 N. Eagleville Rd.
> University of Connecticut
> Storrs, CT 06269
> 860-486-2200
> 860-486-4331 (fax)
>
>
>