Re: Immersion Oil

Posted by S. Brunet on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Immersion-Oil-tp2491973p2493760.html

Hello:

Don't mix Zeiss Immersol 518F and Cargille Type 37 immersion oils (the SOP I
wrote was clear on this!).  I tried cleaning it but I had no luck.  If I put a
drop of Zeiss oil on that objective and put a coverslip on, I can clearly see a
region which does not mix when I image the oil area/volume under a different
microscope!

I clean all objectives between batches of Zeiss oil just in case.

Bye!
Sophie
____________________________________________________
Sophie M. K. Brunet, Ph. D.
Research Officer
Optical Spectroscopy, Laser Systems and Applications
[hidden email]
306-966-1719 (office)   306-966-1702 (fax)
____________________________________________________
Saskatchewan Structural Sciences Centre
University of Saskatchewan
Thorvaldson Bldg.
110 Science Place
Saskatoon, Sk   S7N 5C9
____________________________________________________


Quoting David Knecht <[hidden email]>:

> We now have Nikon, Zeiss and Leica microscopes in our facility.  Is
> the problem of cross-contamination of immersion oil real or
> hypothetical?   Is it possible to use a single type of immersion oil
> for all, so that we don't have to worry about moving samples between
> microscopes?   Dave
>
> Dr. David Knecht
> Department of Molecular and Cell Biology
> Co-head Flow Cytometry and Confocal Microscopy Facility
> U-3125
> 91 N. Eagleville Rd.
> University of Connecticut
> Storrs, CT 06269
> 860-486-2200
> 860-486-4331 (fax)
>
>
>