Confocal Microscopy List

Re: Optical Coupling Gels

Posted by Guy Cox on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Optical-Coupling-Gels-tp2518597p2520157.html

I would have thought (given its intended use) that KY Jelly would be isotonic anyway.

Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
    http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
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Phone +61 2 9351 3176     Fax +61 2 9351 7682
Mobile 0413 281 861
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     http://www.guycox.net

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Cameron Nowell
Sent: Monday, 23 March 2009 3:57 PM
To: [hidden email]
Subject: Re: Optical Coupling Gels

Hi Steve,

That was one we are going to try and probably a lot easier than making polymer gels etc. What would be the easiest way to make it isotonic? Just add some conc saline solution to it?

Thanks

Cam

Cameron J. Nowell
Microscopy Manager
Centre for Advanced Microscopy
Ludwig Institute for Cancer Research
PO Box 2008
Royal Melbourne Hospital
Victoria, 3050
AUSTRALIA

Office: +61 3 9341 3155
Mobile: +61422882700
Fax: +61 3 9341 3104

Facility Website

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Stephen Cody
Sent: Monday, 23 March 2009 3:20 PM
To: [hidden email]
Subject: Re: Optical Coupling Gels

Dear Cam,

Martin Harris (formerly OptiScan now BioPhotonics Australia) used KY jelly for this purpose. Only warning would be if you were using it internally on exposed tissue of the mouse you should make it isotonic. But it should be fine on the skin of a mouse. I shouldn't imagine there would be anything in it to damage the lens either.

All the best,
Steve Cody

Stephen H. Cody
Imaging Research Fellow & Manager
Monash Micro Imaging – AMREP
6 Floor Burnet Tower
Alfred Medical Research & Education Precinct
89 Commercial Rd, Melbourne, Australia, 3004
Phone (Monash): (613) 990 30142
Phone (BakerIDI): (613) 8532 1580

Cameron Nowell wrote:

Hi List,

Has anyone had any experience in using optical gels? We are looking at imaging lymphatic vessels in a mouse tail (still attached to a live mouse) and eventually lymph nodes with our two photon system. The scope is fitted with a 20x water immersion lens (NA 0.95) but we don’t want to submerge the whole tissue. So ideally we need a thick gel that has the same RI of water and won’t damage the objective.

I found one paper (Rothstein et al.) that use sorbitol gelled with carbomer 940 to create a gel with a refractive index similar to water. Is there any other gels that anyone has used before? I was thinking of acrlyamide (polymerised) but cannot find the RI of it.

Cheers

Cam

Cameron J. Nowell
Microscopy Manager
Centre for Advanced Microscopy
Ludwig Institute for Cancer Research
PO Box 2008
Royal Melbourne Hospital
Victoria, 3050
AUSTRALIA

Office: +61 3 9341 3155
Mobile: +61422882700
Fax: +61 3 9341 3104

Facility Website

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