Re: Oil in a 10x objective

Posted by Gary Laevsky-2 on Mar 27, 2009; 6:22pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/Oil-in-a-10x-objective-tp2545827p2545904.html

A great wise one once told me to always store objectives in their case
with back aperture up, so just in case oil didn't drip into the body if
stored back aperture down.

Best,

 

Gary Laevsky, Ph.D.

Imaging Application Specialist

 

Andor Technology

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-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Steven Ruzin
Sent: Friday, March 27, 2009 2:14 PM
To: [hidden email]
Subject: Oil in a 10x objective

Can anyone come up with a plausible explanation as to how oil got
into the 10x objective on our LSM510 with an upright stand? Yesterday
someone complained about poor imaging through the objective, and I
discovered that there was a bubble of oil trapped between internal
elements. I'm at a loss as to how someone got oil inside this low
mag, non-immersion lens. Any ideas?

Steve...
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________________________________________________________________________
____
Steven E. Ruzin, Ph.D.
Director, Biological Imaging Facility
Curator, Golub Microscope Collection
381 Koshland Hall College of Natural Resources
University of California Berkeley CA 94720-3102
510-642-6602 510-642-4995 (fax)
http://golubcollection.berkeley.edu
http://microscopy.berkeley.edu