Posted by
Armstrong, Brian on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Oil-in-a-10x-objective-tp2545827p2545985.html
User focusing on specimen with a 63x/1.4 oil objective, user then
chooses 10x/0.5 from dropdown list in software and depresses the button,
stage drops, nosepiece quickly rotates into position, stage raises, user
then focuses 10x/0.5 objective into immersion oil, user does not bother
to clean oil off of the 10x or tell core manager, core manager does not
bother to check if oil is on the 10x because no-one would put oil on a
10x, right? Oil remains for days/weeks, due to capillary action oil
invades the elements of the objective. Oil is now inside. It's just that
easy.
Or, perhaps I am completely wrong and it was by magic.
Cheers, Brian
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]]
On Behalf Of Steven Ruzin
Sent: Friday, March 27, 2009 11:14 AM
To:
[hidden email]
Subject: Oil in a 10x objective
Can anyone come up with a plausible explanation as to how oil got
into the 10x objective on our LSM510 with an upright stand? Yesterday
someone complained about poor imaging through the objective, and I
discovered that there was a bubble of oil trapped between internal
elements. I'm at a loss as to how someone got oil inside this low
mag, non-immersion lens. Any ideas?
Steve...
--
________________________________________________________________________
____
Steven E. Ruzin, Ph.D.
Director, Biological Imaging Facility
Curator, Golub Microscope Collection
381 Koshland Hall College of Natural Resources
University of California Berkeley CA 94720-3102
510-642-6602 510-642-4995 (fax)
http://golubcollection.berkeley.eduhttp://microscopy.berkeley.edu---------------------------------------------------------------------
SECURITY/CONFIDENTIALITY WARNING:
This message and any attachments are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender.
---------------------------------------------------------------------