Re: Oil in a 10x objective

Posted by Goodhouse, Joseph G. on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Oil-in-a-10x-objective-tp2545827p2546146.html

        In order for the fluid to get into your lens you would also have
to have the lens seal compromised.  Now how does that happen?  I once
had a user drop a 40X plan Apo 1.3 oil objective.  The impact put a dent
in the side of the objective and it cracked the optic. Needless to say
whoever did it did not come forward. Cleaning lenses with organics such
as acetone will compromise the sealant. I have seen this occur.


Joe Goodhouse
Confocal Core Lab Manager
Dept. of Molecular Biology
Princeton University
609-258-5432

Visit us at http://www.molbio1.princeton.edu/facility/confocal/   


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Steven Ruzin
Sent: Friday, March 27, 2009 2:14 PM
To: [hidden email]
Subject: Oil in a 10x objective

Can anyone come up with a plausible explanation as to how oil got into
the 10x objective on our LSM510 with an upright stand? Yesterday someone
complained about poor imaging through the objective, and I discovered
that there was a bubble of oil trapped between internal elements. I'm at
a loss as to how someone got oil inside this low mag, non-immersion
lens. Any ideas?

Steve...
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________________________________________________________________________
____
Steven E. Ruzin, Ph.D.
Director, Biological Imaging Facility
Curator, Golub Microscope Collection
381 Koshland Hall College of Natural Resources
University of California Berkeley CA 94720-3102
510-642-6602 510-642-4995 (fax)
http://golubcollection.berkeley.edu
http://microscopy.berkeley.edu